RESUMO
Four strains of a novel yeast species were isolated from laboratory nests of the leaf-cutting ant Atta sexdens in Brazil. Three strains were found in older sponges and one was in a waste deposit in the ant nests. Sequencing of the D1/D2 region of the large-subunit rRNA gene showed that the novel species, named Sympodiomyces attinorum sp. nov., is phylogenetically related to Sympodiomyces parvus. Unlike Sympodiomyces parvus, Sympodiomyces attinorum can ferment glucose, assimilate methyl alpha-D-glucoside, salicin and citrate, and grow at 37 degrees C, thus enabling these two species to be distinguished. Differentiation from other related species is possible on the basis of other growth characteristics. The type strain of Sympodiomyces attinorum is UNESP-S156T (= CBS 9734T = NRRL Y-27639T).
Assuntos
Formigas/microbiologia , Saccharomycetales/classificação , Saccharomycetales/isolamento & purificação , Animais , Álcoois Benzílicos/metabolismo , Brasil , Ácido Cítrico/metabolismo , Impressões Digitais de DNA , DNA Fúngico/química , DNA Fúngico/isolamento & purificação , DNA Ribossômico/química , DNA Ribossômico/isolamento & purificação , Fermentação , Genes de RNAr/genética , Glucose/metabolismo , Glucosídeos , Metilglucosídeos/metabolismo , Dados de Sequência Molecular , Filogenia , RNA Fúngico/genética , Saccharomycetales/citologia , Saccharomycetales/fisiologia , Análise de Sequência de DNA , TemperaturaRESUMO
The focus of this study was the identification of compounds from plant extracts for use in crop protection. This paper reports on the toxic activity of fractions of leaf extracts of Ricinus communis L (Euphorbiaceae) and isolated active compounds in the leaf-cutting ant Atta sexdens rubropilosa Forel and its symbiotic fungus Leucoagaricus gongylophorus (Singer) Möller. The main compounds responsible for activity against the fungus and ant in leaf extracts of R communis were found to be fatty acids for the former and ricinine for the ants.
